The Choir of Queens' College, Cambridge is a mixed-voice choir comprised of singers drawn from across the University of Cambridge. The choir comprises of Choral Scholars, recruited through the Inter-collegiate Choral Award scheme (all voice parts), and 'volunteers', recruited through auditions which take place within Queens' throughout the year (the main bulk being in October). Over the last decade it has established a reputation for innovative and exciting performances of a very wide range of repertoire. The choir is recruited and directed by two student Organ Scholars (who hold the post for just three years each) ensuring a malleable ensemble adapting constantly to changes of repertory and approach.

The choir's busy term-time schedule includes a twice-weekly Choral Evensong, special events marking the College's year, and services in Cathedrals all over Britain, as well as a lively concert calendar. The choir records regularly, having recently begun successful relationships with two major record companies. The disc Evening Watch was released in January 1999 by Guild Music Ltd, and gained high praise from the journal Choir and Organ, who in a major profile of the choir in its November 1999 issue called it "an exceptional performance of core repertoire". Four further recordings have since been released since, including Flight of Song which achieved playings on Radio Three and Classic FM within a month of its release. The choir's most recent release is Love and Honour (released in January 2005) and it very quickly attracted favourable reviews.

Summer tours of the past few years have included Italy, France, Spain, the Channel Islands and the USA. In 1999, as part of their two-week trip to Germany and Switzerland, the choir sang at the 10th Munich International Chorfest in Munich's prestigious Philharmonie im Gasteig. Most recently the choir toured Hong Kong in December 2007, performing in many prestigious venues, and in partnership with Queen's College Hong Kong. In February 2009 the choir augmented the Philharmonia Chorus in London Lyric Opera's concert performance of Fidelio at London's Cadogan Hall.

Queens' College Choir has been a keen promoter of contemporary music since Charles Villiers Stanford (himself a Queens' Organ Scholar in 1870) wrote a setting of the Magnificat and Nunc Dimittis, The Queens' Service, for the choir, in 1874. Many of the choir's commercial recordings include première recordings, and the Queens' 2000 composition competition attracted entries from all over the world. More recently, the rebuilding of the historic 1892 Binns organ in Queens' College Chapel (in October 2002) and the Accession of Her Majesty the Queen to the role of Patroness of the college (in Spring 2003) have triggered a number of commissions from composer Tarik O'Regan, which feature on the choir's most recent recording, Love and Honour. The choir is currently involved in the three-year Vigani's Cabinet composition project, and last year gave the first performance and recording of works by Michael Finissy and James Weeks.